CDKN1B Antibody

SKU: orb1252597

Description

CDKN1B Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLWestern blot: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.25-0.5 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the p27Kip1 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: KIP1/769
• Immunogen: Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
• Target: CDKN1B
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1

Formalin-fixed, paraffin-embedded human colon carcinoma stained with p27Kip1 antibody (KIP1/769).

Formalin-fixed, paraffin-embedded human prostate cancer stained with p27Kip1 antibody (KIP1/769).

Formalin-fixed, paraffin-embedded human cervical cancer stained with p27Kip1 antibody (KIP1/769).

Flow cytometry testing of Jurkat cells. Black: cells alone; Grey: isotype control; Green: Alexa Fluor 488-labeled p27Kip1 antibody (KIP1/769).

Formalin-fixed, paraffin-embedded rat colon stained with p27Kip1 antibody (KIP1/769).

Quick Database Links

Gene Symbol

CDKN1B

UniProt

P46527