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p27 (phospho Ser10) rabbit pAb Antibody
Description
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p27 Kip1 around the phosphorylation site of Ser10. AA range:1-50 |
| Molecular Weight | 22kD |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western Blot analysis of various cells using Phospho-p27 (S10) Polyclonal Antibody.

Western blot analysis of lysates from A2780 and COLO205 cells, using p27 Kip1 (Phospho-Ser10) Antibody. The lane on the right is blocked with the phospho peptide.
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Protocol Information
p27 (phospho Ser10) rabbit pAb Antibody (orb764253)
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