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p27 KIP 1 Rabbit mAb [KD Validated]

Catalog Number: orb1951759

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb1951759
CategoryAntibodies
DescriptionThe encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
ClonalityRecombinant
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman, Rat
Form/AppearanceRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenA synthesized peptide derived from human p27 KIP 1
UniProt IDP46527
Tested applicationsFC, ICC, IHC, IP, WB
Dilution rangeWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
Antibody TypeRecombinant Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1
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NoteFor research use only
p27 KIP 1 Rabbit mAb [KD Validated]

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

p27 KIP 1 Rabbit mAb [KD Validated]

Immunofluorescent analysis of Hela cells, using p27 KIP 1 Antibody.

p27 KIP 1 Rabbit mAb [KD Validated]

Immunohistochemical analysis of paraffin-embedded human colon, using p27 KIP 1 Antibody.

p27 KIP 1 Rabbit mAb [KD Validated]

Western blot analysis of p27 KIP 1 expression in MCF-7 cell lysate.