p27 KIP 1 Rabbit mAb [KD Validated]

• Catalog Number: orb1951759
• Category: Antibodies
• Description: The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human p27 KIP 1
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P46527
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of Hela cells, using p27 KIP 1 Antibody.

Immunohistochemical analysis of paraffin-embedded human colon, using p27 KIP 1 Antibody.

Western blot analysis of p27 KIP 1 expression in MCF-7 cell lysate.