CDKN1A Antibody

SKU: orb344398

Description

p21 WAF1 antibody

Images & Validation

• Tested Applications: ELISA, IF, IP, Multiplex Assay, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IF: 1:250, IP: 1:100, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-p21/WAF1 has been tested in ELISA, SDS-Page, western blot, and immunofluorescence. This product is also suitable for immunohistochemistry. The antibody detects a 21 kDa band corresponding to the p21 protein. MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry. Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining). In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein. This antibody immunoprecipitates monomeric p21WAF1 and not p21 complexes with other proteins. High level expression of p21WAF1 requires induction of wild-type p53 expression (e.g. via exposure to DNA damaging agents).

  Read more...

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: WA-1
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.
• Target: CDKN1A
• Purity: This protein A purified mouse monoclonal antibody reacts with human and mammalian p21. The intracellular protein p21 is a 21 kD protein and also known as wild-type p53-activated fragment 1 (WAF1). It is an inhibitor of cyclin-dependent kinases (Cdks) and proliferating-cell nuclear antigen (PCNA). The antibody is highly specific and shows no cross-reactivity with other closely related mitotic inhibitors.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-p21/WAF1 antibody, Cyclin-dependent kinase inhibitor 1A (p21, CIP1) antibody, DNA Synthesis Inhibitor antibody, MDA 6 antibody, MDA6 antibody, Melanoma Differentiation Associated Protein 6 antibody

Immunofluorescence of Mouse anti-p21 antibody. Tissue: human brain. Fixation: free-floating. Antigen retrieval: not required. Primary antibody: anti-human-p21 antibody at 1:250 for 1 h at RT. Co-stained with YFP and Sox2 antibodies. Localization: p21 is nuclear and cytoplasmic. Staining: p21 as precipitated blue with Cy5, YFP as precipitated green with Cy2, and Sox2 as precipitated red with Cy3. z‐stacks from confocal expressed as one composite focal plain.

Mab anti-Human p21WAF1 antibody (clone WA-1) is shown to detect human p21 by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate (p/n orb348668) is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human p21WAF1 at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5000. A single band corresponding to human p21WAF1 is detected at ~21 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p21WAF1.

Quick Database Links

Gene Symbol

CDKN1A

UniProt

P38936

RefSeq (Protein):NP_510867.1