p21 WAF1 antibody

• Catalog Number: orb344398
• Category: Antibodies
• Description: p21 WAF1 antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: WA-1
• Tested applications: ELISA, IF, IHC, IP, Multiplex Assay, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IHC: User Optimized, IF: 1:250, IP: 1:100, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This protein A purified mouse monoclonal antibody reacts with human and mammalian p21. The intracellular protein p21 is a 21 kD protein and also known as wild-type p53-activated fragment 1 (WAF1). It is an inhibitor of cyclin-dependent kinases (Cdks) and proliferating-cell nuclear antigen (PCNA). The antibody is highly specific and shows no cross-reactivity with other closely related mitotic inhibitors.
• Conjugation: Unconjugated
• UniProt ID: P38936
• NCBI: NP_510867.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-p21/WAF1 antibody, Cyclin-dependent kin
• Note: For research use only
• Application notes: Anti-p21/WAF1 has been tested in ELISA, SDS-Page, western blot, and immunofluorescence. This product is also suitable for immunohistochemistry. The antibody detects a 21 kDa band corresponding to the p21 protein. MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry. Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining). In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein. This antibody immunoprecipitates monomeric p21WAF1 and not p21 complexes with other proteins. High level expression of p21WAF1 requires induction of wild-type p53 expression (e.g. via exposure to DNA damaging agents).
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa whole cell lysate is loaded per lane using p21 WAF1 antibody