CDKN1A Antibody

SKU: orb1252077

Description

CDKN1A Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Immunofluorescence: 1-2 µg/mLWestern Blot: 1-2 µg/mLImmunohistochemistry (FFPE): 2-4 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the p21 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: WAF21-1
• Immunogen: Recombinant full-length human protein was used as the immunogen for the p21 antibody. This antibody is not suitable for testing mouse and rat samples.
• Target: CDKN1A
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1

IHC testing of human bladder carcinoma and p21 antibody (WAF21-1).

IHC testing of human lung squamous cell carcinoma (SCC) and p21 antibody (WAF21-1).

Western blot testing of human HeLa cell lysate with p21 antibody (clone WAF21-1).

Immunofluorescent staining of human HeLa cells with p21 antibody (clone WAF21-1).

Quick Database Links

Gene Symbol

CDKN1A

UniProt

P38936