Oxt Antibody

• Catalog Number: orb1249165
• Category: Antibodies
• Description: Oxt Antibody
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF
• Predicted Reactivity: Rat
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: DGCRTDPACDPES
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Oxt
• UniProt ID: P35454
• NCBI: NP_035155.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Oxt, oxytocin, OT, Oxy, OTTMUSP00000016535
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 22kDa band in Mouse Liver after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 12.9kDa according to NP_035155.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of NIH3T3 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249165 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and membrane staining.

orb1249165 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).