Ovalbumin Antibody Fluorescein Conjugated

SKU: orb344847

Description

Ovalbumin antibody (FITC)

Images & Validation

• Tested Applications: DOT, ELISA, IF, IP, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IF: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
• Reactivity: Gallus
• Application Notes:
  Anti-Ovalbumin fluorescein conjugated antibody has been tested by ELISA, dot blot, and western blot and is suitable for fluorescent western blot, immunofluorescence microscopy, and flow cytometry or FACS analysis as well as other antibody based fluorescent assays. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Ovalbumin [Hen Egg White]
• Purity: Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Ovalbumin Antibody FITC Conjugation, fluorescein conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2

Dot Blot of Rabbit anti-Ovalbumin (Hen Egg White) Fluorescein Conjugated. Antigen: Ovalbumin. Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, 1.23 ng as indicated. Primary antibody: N/A Secondary antibody: Rabbit anti-Ovalbulmin (Hen Egg White) antibody Fluorescein Conjugated 1:1000 for 60 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637) for 1 hour at RT.

Western Blot of Fluorescein conjugated Rabbit anti-Ovalbumin (Hen Egg white) antibody. Lane 1: Non-Reduced Ovalbumin. Lane 2: Opal Pre-stained Ladder. Lane 3: Reduced Ovalbumin. Load: 0.05 µg per lane. Primary antibody: none. Secondary antibody: Fluorescein Rabbit anti-Ovalbumin antibody at 1:1000 for 60 min at RT. Predicted/Observed size: ~35 kDa.

Western Blot of Rabbit anti-Ovalbumin antibody. Lane 1: ~1 µg Ovalbumin protein reduced. Lane 2: 0.25 µg Ovalbumin protein reduced. Lane 3: ~1 µg Ovalbumin protein non-reduced. Lane 4: 0.25 µg Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4°C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10000 for 1.5 hr at RT. Block: orb348637 buffer overnight at 4°C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin. Other band(s): none.

Quick Database Links

UniProt

P01012

RefSeq:P01012.2