Ovalbumin Antibody Biotin Conjugated

• Catalog Number: orb344924
• Category: Antibodies
• Description: Ovalbumin antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IP, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Ovalbumin [Hen Egg White]
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: Anti-Ovalbumin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivities against Ovalbumin from other sources may occur but have not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P01012
• NCBI: P01012.2
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Ovalbumin Antibody biotin Conjugation,
• Note: For research use only
• Application notes: Anti-Ovalbumin Antibody Biotin Conjugated is tested by ELISA, dot blot, and western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western blot of Biotin conjugated Rabbit Anti-Ovalbumin primary antibody. Lane 1: Ovalbumin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ovalbumin biotin conjugated primary antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.

Western Blot of Rabbit anti-Ovalbumin antibody. Lane 1: ~1 µg Ovalbumin protein reduced. Lane 2: 0.25 µg Ovalbumin protein reduced. Lane 3: ~1 µg Ovalbumin protein non-reduced. Lane 4: 0.25 µg Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4°C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10000 for 1.5 hr at RT. Block: orb348637 buffer overnight at 4°C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin. Other band(s): none.