SERPINB14 Antibody (Biotin)

SKU: orb344924

Description

Ovalbumin antibody (Biotin)

Images & Validation

• Tested Applications: DOT, ELISA, IP, WB
• Dilution Range: ELISA: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:500 - 1:2,500
• Reactivity: Gallus
• Application Notes:
  Anti-Ovalbumin Antibody Biotin Conjugated is tested by ELISA, dot blot, and western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Ovalbumin [Hen Egg White]
• Target: SERPINB14
• Purity: Anti-Ovalbumin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivities against Ovalbumin from other sources may occur but have not been specifically determined.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Ovalbumin Antibody biotin Conjugation, biotin conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2

Western blot of Biotin conjugated Rabbit Anti-Ovalbumin primary antibody. Lane 1: Ovalbumin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ovalbumin biotin conjugated primary antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.

Western Blot of Rabbit anti-Ovalbumin antibody. Lane 1: ~1 µg Ovalbumin protein reduced. Lane 2: 0.25 µg Ovalbumin protein reduced. Lane 3: ~1 µg Ovalbumin protein non-reduced. Lane 4: 0.25 µg Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4°C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10000 for 1.5 hr at RT. Block: orb348637 buffer overnight at 4°C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin. Other band(s): none.

Quick Database Links

Gene Symbol

SERPINB14

UniProt

P01012

RefSeq:P01012.2