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OTC Antibody (Center)
Description
Research Area
Images & Validation
−| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:2000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Porcine |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 39935 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

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Flow cytometric analysis of ATDC5 cells using OTC Antibody (Center) (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatoarcinoma with OTC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-OTC Antibody (Center) at 1:2000 dilution. Lane 1: Human liver lysate. Lane 2: Mouse liver lysate. Lane 3: Rat liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
OTC Antibody (Center) (orb1929708)
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