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| Catalog Number | orb1247164 |
|---|---|
| Category | Antibodies |
| Description | ORP5 Antibody |
| Target | OSBPL5 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Canine, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 500 ug/mL |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Immunogen | The immunogen for this antibody is: KEEAFLRRRFSLC |
| UniProt ID | Q9H0X9 |
| Tested applications | ELISA, IHC, WB |
| Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Spleen lysates (calculated MW of 98.4kDa according to NP_065947.1). Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney and Liver. Recommended concentration: 5ug/ml. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | OSBPL5, ORP5, OBPH1, FLJ31948, KIAA1534, DKFZp586H Read more... |
| Research Area | Cancer Biology |
| Note | For research use only |
| NCBI | NP_663613.1, NP_065947.1, NP_001137535.1 |

orb1247164 (0.2 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247164 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247164 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, FC, IF, IHC, WB | |
Canine, Mouse | |
Human, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P | |
Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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