Ornithine Decarboxylase Antibody / ODC1

• Catalog Number: orb639842
• Category: Antibodies
• Description: Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human ODC-1 protein was used as the immunogen for this recombinant ODC1 antibody.
• UniProt ID: P11926
• Tested applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 0.25-0.5ug/ml,Western blot: 0.5-1ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rODC1/485
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant ODC1 antibody is available for research use only.
• Note: For research use only

Western blot testing of human PC-3 cell lysate with recombinant ODC1 antibody. Predicted molecular weight ~51 kDa.

IHC staining of FFPE human prostate carcinoma with recombinant ODC1 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant ODC1 antibody (clone rODC1/485) as confirmation of integrity and purity.