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Catalog Number | orb1239690 |
---|---|
Category | Antibodies |
Description | op18 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Predicted Reactivity | Bovine, Gallus, Porcine |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Anti-op18 antibody (orb1239690) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human op18. The immunogen is located within the first 50 amino acids of op18. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 17kDObserved: 17 kD |
Target | STMN1 |
UniProt ID | P16949 |
NCBI | AAH82228 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | op18 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | op18 Antibody: Lag, SMN, OP18, PP17, PP19, PR22, L Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot Validation in Human Brain Tissue. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239690 (A: 0.5 µg/mL, B: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239686 (2 µg/mL), op18 orb1239690 (2 µg/mL), and GAPDH orb1239765 (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239690 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239690 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of op18 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-op18 antibody (orb1239690) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of op18 in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Brain Tissue labeling op18 with orb1239690 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
ELISA, ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P | |
Bovine, Gallus, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Bovine, Gallus, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |