Olomoucine

SKU: orb1306287

Description

Olomoucine is a cell-permeable purine analog that acts as an ATP-competitive inhibitor of multiple cyclin-dependent kinases (CDKs) and ERK1, with IC50 values ranging from 3 to 25 µM. It is widely used in biochemical and cellular research to study cell cycle regulation, signal transduction, and has demonstrated anti-proliferative effects in melanoma models both in vitro and in vivo.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 101622-51-9
• MW: 298.34
• Purity: 99.80%
• Formula: C₁₅H₁₈N₆O
• SMILES: Cn1cnc2c(NCc3ccccc3)nc(NCCO)nc12
• Target: ERK,CDK
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.7 mM);DMSO:50 mg/mL (167.59 mM)

Bioactivity

• Target IC50:
  CDK5-p35:25 μM|CDK2-CyclinA:7 μM|CDK2-CyclinE:7 μM
• In Vivo:
  In female Balb C− mice, Olomoucine results faster plasma concentration decreasing with 50 mg/kg (as single agent) than 16.6 mg/kg (as cassette). In dog with spontaneous melanoma, Olomoucine (8 mg/kg; i.v.) induces programmed cell death of cancer cells and results in rapid eradication of at least 68% of the tumor cells without side effects.
• In Vitro:
  In Rdditapes oocytes, Olomoucine (0-150 μM) inhibits prophase/metaphase transition. In CTLL-2 cells, Olomoucine (0-1000 μM) inhibits DNA synthesis and triggers a Gl arrest similar to interleukin-2 deprivation. In NSCLC MB65 cells, Olomoucine (0-100 μM) inhibits Gl/S transition. Olomoucine inhibits tumor cell survival with IC50s of 32.35, 42.15, and 82.30 μM for dog, mouse B16, and human melanoma, respectively.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cell cycle, CDK5/p35, CDK, cdk2, CDK2/CyclinE, cdc2, Cdc2/CyclinA, Cdc2/CyclinB, cyclindependent kinases, Cyclin dependent kinase, non-small cell lung cancer cell line, inhibit, Inhibitor, MR6.5, Olomoucine, ERK, purines, xenopus laevis oocytes, protein kinases, proliferation

Compound Identifiers

PubChem CID

4592