Oleic acid

SKU: orb1304743

Description

Oleic acid (Glycon Wo) is a common monounsaturated fatty acid naturally present in many animal and vegetable fats. It functions as an activator of the Na+/K+ ATPase enzyme and is widely used in biochemical research to study ion transport mechanisms in both cellular and physiological models.

Research Area

Cell Biology, Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 112-80-1
• MW: 282.46
• Purity: 99.91%
• Formula: C₁₈H₃₄O₂
• SMILES: C(C/C=C\CCCCCCCC)CCCCCC(O)=O
• Target: ATPase,Endogenous Metabolite,Apoptosis
• Solubility: 5% DMSO+95% Saline:3 mg/mL (10.62 mM);DMSO:250 mg/mL (885.08 mM)

Bioactivity

• Target IC50:
  FFA1/GPR40:58.4 μM (EC50)|GPR3:300 μM (EC50)|W83 cells:1.55 nM|gastric H+, K (+)-ATPase:12 μM|Porcine Pancreatic Lipase:11.7 µg/mL (41.4µM)
• In Vivo:
  METHODS: To detect the antitumor activity in vivo, Oleic acid (10 mg/kg) was administered by gavage to LKB1fl/flp53fl/fl endometrial tumor mouse model five days per week for four weeks. RESULTS: Oleic acid treatment for four weeks significantly inhibited tumor growth by 52.1%.Oleic acid exhibits anti-tumor activity in endometrial cancers, which is dependent on the PTEN/AKT/mTOR signaling pathway. METHODS: To study its role in ischemic injury, Oleic acid (10-30 mg/kg, 5% Tween 20 in sterile saline) was administered intraperitoneally to rodent models of middle cerebral artery occlusion (MCAO), photochromic and four-vessel occlusion (4-VO). RESULTS: Oleic acid administration reduced MCAO-induced infarct volume and functional deficits, photochromic-induced infarct volume, and 4-VO-induced hippocampal neuronal death.
• In Vitro:
  METHODS: Human tumor cells HCG-27, AGS, MDA-MB-231, SGC7901, BGC823 and MCF-7 were treated with Oleic acid (400 μM) for 24-72 h. Cell viability was detected using MTT. RESULTS: Oleic acid stimulated cell viability in HGC-27, MDA-MB-231 cells, and inhibited cell viability in AGS, SGC7901, BGC823 and MCF-7 cells. METHODS: Renal cell carcinoma cells 786-O were treated with Oleic acid (0.05-0.2 mM/L) for 48 h. Apoptosis was detected by Flow Cytometry. RESULTS: Oleic acid retarded apoptosis of 786-O cells in a concentration-dependent manner.

Storage & Handling

• Storage: store at low temperature | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Na+/K+ ATPase, Oleate, Oleic acid, Elaidoic acid, EndogenousMetabolite, Endogenous Metabolite, Glycon Wo, Inhibitor, Apoptosis, 9-cis-Octadecenoic acid, 9-Octadecenoic Acid, 9Z-Octadecenoic acid, Sodium potassium pump

Compound Identifiers

PubChem CID

445639