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Catalog Number | orb1256615 |
---|---|
Category | Antibodies |
Description | OGFR Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human OGFR (NP_031372.2). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 110kDa |
Target | OGFR |
UniProt ID | Q9NZT2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | OGFR, opioid growth factor receptor, HGNC:15768, 7 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using OGFR antibody (orb1256615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using OGFR antibody (orb1256615) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using OGFR antibody (orb1256615) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using OGFR antibody (orb1256615) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using OGFR antibody (orb1256615). Blue: DAPI for nuclear staining.