OGDH Recombinant Mouse Monoclonal Antibody

SKU: orb1172532

Description

OGDH Recombinant Mouse Monoclonal Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:50-200
• Reactivity: Human
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG2b/Kappa
• Clone No.: F8B3
• Target: OGDH
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC; OGDH-E1; OGDH2; OGDHD; 2210403E04Rik; 2210412K19Rik; d1401; mKIAA4192; ODO1_BOVIN; OGDH; OGDC-E1; 2-oxoglutarate dehydrogenase, mitochondrial; Alpha-ketoglutarate dehydrogenase (Alpha-KGDH-E1); Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase; 1.2.4.2; ODO1_HUMAN; ODO1_MOUSE; Kiaa4192; ODO1_RAT; Ogdhl;

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: room temperature 2h, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: Raji, 2: HepG2, 3: MCF-7, 4: Rat heart, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 116 kDa, Observed MW: 116 kDa.

Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172532.

Quick Database Links

Gene Symbol

OGDH