ODZ3 Antibody

• Catalog Number: orb1246513
• Category: Antibodies
• Description: ODZ3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-NNPASKIHDVKDY
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments in Human Brain lysates and in lysates of cell line NIH 3T3 gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Brain (Cerebellum) shows nuclear staining of the granular cell layer. Recommended concentration, 1.5-3ug/ml.Immunofluorescence: Nuclear staining (green) in HeLa cells.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ODZ3
• UniProt ID: Q9P273
• NCBI: NP_001073946.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ODZ3, odz, odd Oz/ten-m homolog 3 (Drosophila), FL
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments in Human Brain lysates and in lysates of cell line NIH 3T3 gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Brain (Cerebellum) shows nuclear staining of the granular cell layer. Recommended concentration, 1.5-3ug/ml.Immunofluorescence: Nuclear staining (green) in HeLa cells.
• Expiration Date: 12 months from date of receipt.

orb1246513 (10 ug/ml) staining of nuclei HeLa cells (green). Detected by immunofluorescence.

orb1246513 (1.5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.