OD36

SKU: orb1694395

Description

OD36 is a potent, selective small molecule inhibitor of RIPK2 (IC50 = 5.3 nM) and ALK2 (KD = 37 nM), effectively occupying their ATP-binding sites. It is a valuable research tool for studying innate immunity, heterotopic ossification, and BMP signaling pathways in cellular models.

Research Area

Cardiovascular Research, Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1638644-62-8
• MW: 330.77
• Purity: 99.89% (May vary between batches)
• Formula: C₁₆H₁₅ClN₄O₂
• SMILES: Clc1cc2OCCOCCN=C3Nc4c(cnn4C=C3)-c(c1)c2
• Target: RIP kinase,ALK,TGF-beta/Smad
• Solubility: DMSO:25 mg/mL (75.58 mM)

Bioactivity

• Target IC50:
  ALK2 (R206H):22 nM (IC50)|RIPK2:5.3 nM (IC50)|ALK2:37 nM (Kd)|ALK2:47 nM (IC50)
• In Vivo:
  OD36 (6.25 mg/kg; i.p.; once; C57BL/6 mice, muramyl dipeptide (MDP)-induced model of peritonitis) inhibited the recruitment of inflammatory cells to the peritoneum, specifically neutrophils, and to a lesser extent, lymphocytes. It decreased RIPK2-specific genes, as well as inflammatory cytokine and chemokine gene expression. OD36 alleviates inflammation in an acute peritonitis mice model.
  Read more...
• In Vitro:
  OD36 shows activity against ALK1 (KD=90 nM). OD36 potently antagonizes mutant ALK2 signaling and osteogenic differentiation. OD36 (0.1-1 μM; 24 h; KS483 cells) efficiently inhibits BMP-6 (50 ng/mL)-induced p-Smad1/5. OD36 (0.5 μM) completely prevents the activation of Smad1/5 and the gene targets ID-1 and ID-3 in response to activin A in preincubated fibrodysplasia ossificans progressiva (FOP) endothelial colony-forming cells (ECFCs).
  Read more...

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALK2, OD36, OD-36, OD36 Hydrochloride, OD-36 Hydrochloride, OD 36, RIP kinase, RIPK2, RIPkinase

Compound Identifiers

PubChem CID

135567262