OCLN/Occludin Antibody

• Catalog Number: orb1537178
• Category: Antibodies
• Description: OCLN/Occludin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A 15 AA synthetic peptide near the C-Terminus of human OCLN. The immunogen is located within the last 50 AA of OCLN.
• Concentration: 1 mg/ml
• Dilution range: ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
• Conjugation: Unconjugated
• Target: OCLN / Occludin
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Alternative names: OCLN, Occludin, BLCPMG
• Note: For research use only
• Application notes: Further information: OCLN antibody can be used for detection of OCLN by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 57 kDa Observed: 57 kDa
• Expiration Date: 12 months from date of receipt.

Human Thryoid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence of OCLN in human liver tissue with OCLN antibody at 20 µg/ml.

Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 µg/mL.