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Catalog Number | orb1257145 |
---|---|
Category | Antibodies |
Description | OAT Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IP: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 47kDa |
Target | OAT |
UniProt ID | P04181 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | OAT, DKFZp781A11155, HOGA, OKT, GACR, OATASE Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using OAT antibody (orb1257145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug OAT antibody (orb1257145). Western blot was performed from the immunoprecipitate using OAT antibody (orb1257145) at a dilution of 1:1000.
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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