OAS2 Antibody

• Catalog Number: orb1248212
• Category: Antibodies
• Description: OAS2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: QPNKCFLEQIDSAVN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell lines Daudi, Jurkat and K562 (calculated MW of 78.8kDa according to NP_002526.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lin et al, J Immunol. 2009 Dec 15;183(12):8035-43. PMID: 19923450). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 70kDa
• Target: OAS2
• UniProt ID: P29728
• NCBI: NP_058197.2, NP_002526.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: OAS2, 2'-5'-oligoadenylate synthetase 2, 69/71kDa,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell lines Daudi, Jurkat and K562 (calculated MW of 78.8kDa according to NP_002526.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lin et al, J Immunol. 2009 Dec 15;183(12):8035-43. PMID: 19923450). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248212 (0.3 ug/ml) staining of Daudi (A), Jurkat (B) and K562 (C) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248212 (5 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.