OAC2

SKU: orb1306728

Description

OAC2

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 6019-39-2
• MW: 236.27
• Purity: 99.76%
• Formula: C₁₅H₁₂N₂O
• SMILES: O=C(Nc1ccc2[nH]ccc2c1)c1ccccc1
• Target: OCT
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (8.46 mM);DMSO:23.6 mg/mL (99.89 mM)

Bioactivity

• In Vitro:
  Octamer-binding transcription factor 4 (Oct4) is crucial for initiating and maintaining cellular pluripotency, especially during early differentiation stages, and cannot be substituted by any family members for this function. Essential for embryonic stem cell (ESC) pluripotency and reprogramming, Oct4, along with Sox2, Klf4, and c-Myc, benefits from the structural analog OAC2, which activates Oct4 and Nanog reporters like OAC1. OAC1 and its analogs OAC2 and OAC3 significantly enhance reprogramming efficiency by up to 2.75% and accelerate iPSC colony formation by 3 to 4 days.
• Cell Research:
  The Oct4-luc or Nanog-luc cells are treated with compound OAC1 or its structural analogs OAC2, OAC3 at 1 μM concentration or at indicated concentrations. Other compounds used include 2 μM BIO, 2 μM BIX, 2 μM 5'-azacytidine, 25 μg/mL Vitamin C, 10 nM Am580, 5 μM tranylcypromine, and 0.5 mM valporic acid. Luciferase reporter assays are performed 24 h after compound treatment or at indicated time points. For Topflash reporter assays, 0.2 μg β-catenin–responsive Topflash reporter gene plasmid is introduced into CV1 cells using trasfection. Compounds are added 6 h after transfection. Luciferase activity is measured 48 h after compound treatment using the Glo Luciferase Assay System.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, OAC 2, OAC2, OAC-2, Oct3/4, Inhibitor, Slc22a3, POU5F1

Compound Identifiers

PubChem CID

2814138