O-GlcNAc Antibody: HRP

• Catalog Number: orb396437
• Category: Antibodies
• Description: Mouse monoclonal antibody against O-GlcNAc conjugated to HRP.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9H6
• Tested applications: ELISA, IHC, WB
• Reactivity: All
• Isotype: IgM
• Immunogen: Synthetic β-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25)
• Conjugation: HRP
• Target: O-GlcNAc
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: β-O-Linked N-Acetylglucosamine antibody, O-Linked
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-502 was sufficient for detection of O-GlcNAc in 2 µg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite.

Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa O-GlcNAc protein using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.