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Catalog Number | orb396442 |
---|---|
Category | Antibodies |
Description | Mouse Anti-β-O-Linked N-Acetylgalactosamine Monoclonal IgM |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 9B9 |
Tested applications | ELISA, ICC, IHC, WB |
Reactivity | All |
Isotype | IgM |
Immunogen | Synthetic β-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH). |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC-P (1:25) |
Form/Appearance | PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated |
Purity | PEG Purified |
Conjugation | Unconjugated |
Target | Specific for β-O-Linked N-Acetylgalactosamine and β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins. |
Storage | -20ºC, Conjugated antibodies should be stored at 4ºC |
Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated |
Alternative names | GalNAC Antibody, O-GalNAC Antibody, β-O-Linked N-A Read more... |
Note | For research use only |
Application notes | A 1:1000 dilution of SMC-503 was sufficient for detection of O-GlcNAc in 2 µg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. |
Expiration Date | 12 months from date of receipt. |
Immunocytochemistry analysis of colon tissue using O-GalNAC antibody
Western Blot analysis of ane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 µg using O-GalNAc Antibody
Immunocytochemistry analysis of Embryonic kidney epithelial cell line (HEK293) tissue using O-GalNAC antibody
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