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Catalog Number | orb1931596 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB2874 |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 70538 Da |
Target | This NYREN18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-117 amino acids from the N-terminal region of human NYREN18. |
UniProt ID | Q9Y5A7 |
NCBI | NP_001230280.1, NP_057202.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | NEDD8 ultimate buster 1, Negative regulator of ubi Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-NYREN18 N-term Pab is used in Western blot to detect NYREN18 in mouse liver lysate.
NYREN18 Antibody (E102) western blot analysis in SK-BR-3 cell line lysates (35 ug/lane). This demonstrates the NYREN18 antibody detected the NYREN18 protein (arrow).
Western blot analysis of NUB1 (arrow) using NYREN18 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUB1 gene (Lane 2).