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Item 1 of 4
Item 1 of 4
| Catalog Number | orb464231 |
|---|---|
| Category | Antibodies |
| Description | Nurr1 antibody (FITC) |
| Target | Nurr1 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | FITC |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Equine, Mouse, Porcine, Rat, Sheep |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | Liquid |
| Purification | affinity purified by Protein A |
| Immunogen | KLH conjugated synthetic peptide derived from human Nurr1 |
| UniProt ID | P43354 |
| Tested applications | FC |
| Dilution range | Flow-Cyt=1μg/Test,IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Alternative names | Nur-ralated factor 1; Nuclear receptor-related fac Read more... |
| Note | For research use only |
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Giuliano Bonfá, Giovanna Martino, View ORCID ProfileAssunta Sellitto, Antonio Rinaldi, Fabiana Tedeschi, View ORCID ProfileFabio Caliendo, Loris Melchiorri, Daniela Perna, Freddie Starkey, View ORCID ProfileEvangelos Nikolados, View ORCID ProfileFilippo Menolascina, View ORCID ProfileVelia Siciliano Design of novel synthetic promoters to tune gene expression in T cells bioRxiv, (2025)
Applications
IF
Reactivity
Human
Zhang, Wencan et al. Steroid nuclear receptor coactivator 2 controls immune tolerance by promoting induced Treg differentiation via up-regulating Nr4a2 Sci Adv, 8, (2022)
Applications
FC
Reactivity
Mouse
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (orb464231), dilution: 1 ug/Test, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (orb464231), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: U-87MG. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (orb464231), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
