NR4A1 Antibody

SKU: orb1247182

Description

NR4A1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Thyroid gland lysates (calculated MW of 64.5kDa according to NP_002126.2 and NP_775180.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 5ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-GRLPSKPKQPPDAS
• Target: NR4A1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

NR4A1, NUR77, nuclear receptor subfamily 4, group A, member 1 , GFRP1, HMR, MGC9485, N10, NAK-1, NGFIB, NP10, TR3 , TR3 orphan receptor, early response protein NAK1, growth factor-inducible nuclear protein N10, hormone receptor, orphan nuclear receptor HMR, steroid receptor TR3

orb1247182 (2 ug/ml) staining of Human Thyroid lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247182 (5 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

NR4A1

UniProt

P22736

RefSeq (Protein):NP_002126.2, NP_775180.1