NUPR1 Rabbit Polyclonal Antibody

• Catalog Number: orb101189
• Category: Antibodies
• Description: NUPR1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Rat, Sheep
• Reactivity: Human, Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human NUPR1/p8 (31-82/82aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 9 kDa
• Target: NUPR1
• UniProt ID: O60356
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Candidate of metastasis 1; COM1; Nuclear protein 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Xiaoguang Wang, Ke Wang, Xiuli Wang NUPR1 contributes to activate TFE3-dependent autophagy leading to cervical cancer proliferation Jan 12, Heliyon, 10(2) (2024)

PubMed: 38298693

Applications

IF

B Liu, WZ Deng, WH Hu, RX Lu, QY Zhang, CF Gao, XJ Huang, WG Liao, J Gao, Y Liu Psychological stress-activated NR3C1/NUPR1 axis promotes ovarian tumor metastasis Acta Pharmaceutica Sinica B, (2025)

PubMed:

Applications

IHC

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NUPR1) Polyclonal Antibody, Unconjugated (orb101189) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human L-O2 cell lysates, Lane 2: Human U87MG cell lysates, Primary: Anti-NUPR1 (orb101189) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 9 kDa, Observed band size: 11 kDa.