NUP43 Polyclonal Antibody

• Catalog Number: orb1418058
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NUP43.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Rat
• UniProt ID: Q8NFH3
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nucleoporin Nup43, Nup107-160 subcomplex subunit N
• Note: For research use only

Sample: Hela (Human) Cell Lysate at 30 ug. 293T (Human) Cell Lysate at 30 ug. Primary: Anti-NUP43 at 1/500 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 30 kD.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NUP43) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.