NUDT15 Rabbit pAb Discontinued

SKU: orb374080

Description

Rabbit polyclonal antibody to NUDT15

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human NUDT15 (NP_060753.1).
• Molecular Weight: 19kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NUDT15, MTH2, NUDT15D

Immunohistochemistry analysis of paraffin-embedded Human liver using NUDT15 Rabbit pAb (orb374080) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Human stomach using NUDT15 Rabbit pAb (orb374080) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western blot analysis of various lysates using NUDT15 Rabbit pAb (orb374080) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Quick Database Links

UniProt

Q9NV35

Li, Jin et al. MutT-related proteins are novel progression and prognostic markers for colorectal cancer Oncotarget, 8, 105714-105726 (2017)

PubMed: 29285286