Recombinant Nucleophosmin Antibody / NPM1

SKU: orb639834

Description

Nucleophosmin (NPM) is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml (not suitable for frozen tissue sample testing),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells in 0.1ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant Nucleophosmin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rNPM1/1901
• Immunogen: A recombinant human partial protein (amion acids 185-287) was used as the immunogen for this recombinant Nucleophosmin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Nucleophosmin antibody (clone rNPM1/1901); Red=isotype control, Blue= recombinant Nucleophosmin antibody.

Western blot testing of human K562 cell lysate with recombinant Nucleophosmin antibody. Predicted molecular weight ~38 kDa.

IHC staining of FFPE human skin with recombinant Nucleophosmin antibody (clone rNPM1/1901). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skin with recombinant Nucleophosmin antibody (clone rNPM1/1901). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Nucleophosmin antibody (clone rNPM1/1901) as confirmation of integrity and purity.

Quick Database Links

UniProt

P06748