Nucleolin (Marker of Human Cells) Antibody

SKU: orb388511

Description

Mouse monoclonal antibody to NCL

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution range: FACS: 0.5-1 μg/million cells, IF/ICC: 0.5-1 μg/ml, WB: 0.5-1 μg/ml, IHC-P: 0.25-0.5 μg/ml
• Reactivity: Human
• Application Notes:
  Nucleolin (Marker of Human Cells)

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 364-5
• Immunogen: lysate of SU-DHL-1 Nuclei
• Target: NCL
• Source/Expression System: Bioreactor
• Molecular Weight: 76kDa
• Purification: Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

NCL; Nucl; Nucleolin; Protein C23

Immunohistochemical staining of human Skin tissue using NCL antibody

Immunohistochemical staining of human Tonsil tissue using NCL antibody

Immunohistochemical staining of human Colon carcinoma tissue using NCL antibody

Flow cytometric analysis of 293T Cells using NCL antibody

Quick Database Links

Gene Symbol

NCL

UniProt

P19338