Nucleoli Marker Antibody [NCM102-1]

• Catalog Number: orb1252106
• Category: Antibodies
• Description: Nucleoli Marker Antibody [NCM102-1]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NCM102-1
• Tested applications: FC, ICC, IF, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A preparation of myeloid leukemia cell nuclei was used as the immunogen.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 0.5-1.0 ug/mlImmunocytochemistry (Acetone-fixed): 0.5-1.0 ug/ml for 30 min at RTIHC (Formalin-paraffin): 0.5-1.0 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the Nucleoli marker antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 0.5-1.0 ug/mlImmunocytochemistry (Acetone-fixed): 0.5-1.0 ug/ml for 30 min at RTIHC (Formalin-paraffin): 0.5-1.0 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the Nucleoli marker antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of Nucleoli marker antibody and FFPE human tonsil tissue.

Immunofluorescent testing of Alexa Fluor 488 conjugated Nucleoli marker antibody and human colon carcinoma.

Intracellular FACS testing of Nucleoli marker antibody (green) and 293 cells.