Rorc Antibody

SKU: orb420342

Description

Nuclear receptor ROR gamma (phospho-S203) antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, KO/KD Validated, WB
• Dilution Range: ELISA: 5 ug/mL, WB: 1 ug/mL
• Reactivity: Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 61 kDa in size corresponding to receptor ROR gamma by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Affinity purified Anti-receptor ROR gamma pS203 antibody was prepared from whole rabbit serum produced by repeated immunizations with an internal synthetic peptide phosphorylated at the serine 203 position of mouse receptor ROR gamma protein.
• Target: Rorc
• Purity: Anti-nuclear receptor ROR gamma pS203 is directed against mouse receptor ROR gamma phosphorylated at the serine 203 position. This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide to remove any unwanted reactivities. A BLAST analysis was used to suggest reactivity with this protein with pongo abelii based on 100% homology for the immunogen sequence.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.05 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-receptor ROR gamma pS203 antibody, Nuclear receptor ROR-gamma, Nr1f3, RAR-related orphan receptor C, Nuclear receptor RZR-gamma, Thymus orphan receptor, Nuclear receptor subfamily 1 group F member 3, Retinoid-related orphan receptor-gamma, TOR, Thor, Rorg, Rorc

Western Blot of Rabbit anti-receptor ROR gamma pS203 antibody. Lane 1: Opal PreStained Ladder. Lane 2: Wild-type Mouse Thymus. Lane 3: KO Mouse Thymus. Load: 10 µg per lane. Primary antibody: Receptor ROR gamma pS203 antibody at 1 ug/ml for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 minutes at RT. Predicted/Observed size: 63 kDa for ROR gamma.

Quick Database Links

Gene Symbol

Rorc

UniProt

P51450

RefSeq (Protein):NP_001280663.1