Nuclear Marker Antibody [NM106]

• Catalog Number: orb1252389
• Category: Antibodies
• Description: Nuclear Marker Antibody [NM106]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NM106
• Tested applications: FC, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1, kappa
• Immunogen: Nuclei of HL60 cells were used as the immunogen for the Nuclear marker antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunocytochemistry (Acetone-fixed): 0.25-0.5 ug/ml for 30 min at RTImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Nuclear marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• UniProt ID: NotKnown
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunocytochemistry (Acetone-fixed): 0.25-0.5 ug/ml for 30 min at RTImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Nuclear marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human tonsil stained with Nuclear marker antibody (NM106).

Formalin-fixed, paraffin-embedded human tonsil stained with Nuclear marker antibody (NM106).

Formalin-fixed, paraffin-embedded rat colon stained with Nuclear marker antibody (NM106).

Formalin-fixed, paraffin-embedded rat lung stained with Nuclear marker antibody (NM106).

SDS-PAGE Analysis of Purified, BSA-Free Nuclear Marker Antibody (clone NM106). Confirmation of Integrity and Purity of the Antibody.