Nuclear Marker Antibody [NM106]

SKU: orb1252389

Description

Nuclear Marker Antibody [NM106]

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunocytochemistry (Acetone-fixed): 0.25-0.5 µg/mL for 30 min at RTImmunohistochemistry (FFPE): 0.25-0.5 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Nuclear marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: NM106
• Immunogen: Nuclei of HL60 cells were used as the immunogen for the Nuclear marker antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human tonsil stained with Nuclear marker antibody (NM106).

Formalin-fixed, paraffin-embedded human tonsil stained with Nuclear marker antibody (NM106).

Formalin-fixed, paraffin-embedded rat colon stained with Nuclear marker antibody (NM106).

Formalin-fixed, paraffin-embedded rat lung stained with Nuclear marker antibody (NM106).

SDS-PAGE Analysis of Purified, BSA-Free Nuclear Marker Antibody (clone NM106). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

NotKnown