NTN1/Netrin 1 Antibody

• Catalog Number: orb1538670
• Category: Antibodies
• Description: NTN1/Netrin 1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P
• Reactivity: Bovine, Human, Monkey, Mouse, Rat, Xenopus, Zebrafish
• Immunogen: Peptide with sequence KFQQREKKGKCKKA, from the C-Terminus of protein sequence according to NP_004813.2.
• Concentration: 0.5 mg/ml
• Dilution range: IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000)
• Conjugation: Unconjugated
• Target: NTN1 / Netrin 1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: NTN1, Netrin 1, Netrin 1, mouse, homolog of, Netri
• Note: For research use only
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx. 70kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 67.7kDa according to Human NP_004813.1 and 67.8kDa according to Rat NP_446183.1). Recommended concentration: 0.5-1.5 ug/ml.
• Expiration Date: 12 months from date of receipt.

Anti-Netrin 1 antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

Goat Anti-Netrin 1 Antibody Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).