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Catalog Number | orb1938115 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB17473 |
Tested applications | WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 34390 Da |
Target | This NTHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human NTHL1. |
UniProt ID | P78549 |
NCBI | NP_002519.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Endonuclease III-like protein 1 {ECO:0000255|HAMAP Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-NTHL1 Antibody (Center R103) at 1:1000 dilution. Lane 1: DU145 whole cell lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
NTHL1 Antibody (Center R103) western blot analysis in Hela, NCI-H460 cell line lysates (35 ug/lane). This demonstrates the NTHL1 antibody detected the NTHL1 protein (arrow).