Nsg1 Antibody

SKU: orb1248655

Description

Nsg1 Antibody

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 22kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9kDa according to NP_035072.2). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of EB11637 (red) in the neurons only. Recommended concentration: 1-5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: EKGTKQPLLEDGFD-C
• Target: Nsg1
• Molecular Weight: Approx 22 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

Nsg1, neuron specific gene family member 1, Bsmrb, Neep21, PEP1, brain neuron cytoplasmic protein 1/2, brain specific mRNA b, neuron-specific protein family member 1

orb1248655 (0.1 ug/ml) staining of fetal Mouse Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248655 (3 ug/ml) staining of primary DIV9 cells from Hippocampus E18 Rat embryos showed exclusive localization (red, Alexa 568) within the neurons (MAP2 staining in blue, Alexa 647) and not in the glia (GFAP staining in green, Alexa 488).

Quick Database Links

Gene Symbol

Nsg1

UniProt

P42857

RefSeq (Protein):NP_077042.2