NS-2028

SKU: orb1218504

Description

NS-2028 is a highly selective soluble Guanylyl Cyclase (sGC) inhibitor (IC50: 30 nM and 200 nM for basal and NO-stimulated enzyme activity). It is commonly used in the research of nitric oxide signaling pathways. NS-2028 inhibits soluble Guanylyl Cyclase activity in homogenates of mouse cerebellum and neuronal NO synthase (IC50: 17 nM and 20 nM). NS-2028 inhibits 3-morpholino-sydnonimine (SIN-1)-elicited formation of cyclic GMP in human cultured umbilical vein endothelial cells (IC50: 30 nM). NS-2028 inhibits NO-dependent relaxant responses in non-vascular smooth muscle completely (1 μM). NS-2028 decreases vascular endothelial growth factor-induced angiogenesis and permeability.

Key Properties

• CAS Number: 204326-43-2
• MW: 269.05
• Purity: >98% (HPLC)
• Formula: C₉H₅BrN₂O₃
• SMILES: O=C1ON=C2COC3=CC=C(Br)C=C3N21
• Target: sGC
• Solubility: DMSO: 250 mg/mL (929.20 mM; Need ultrasonic)

Bioactivity

• In Vivo:
  Animal model: Rabbit. Dosage: 1 g/L. Administration: Deliver orally; 1 g/L; 8 days. Result: Inhibits VEGF-induced angiogenesis In vivo.
• In Vitro:
  Cell Proliferation Assay Cell line: HUVEC cells. Concentration: 10 μM. Incubation time: 24 hours. Result: Decreased cell numbers in culture. Western blot analysis. Cell line: HUVEC cells. Concentration: 10 μM. Incubation time: 30 mins. Result: Attenuated VEGF-enhanced p38 phosphorylation.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

sGC