NR3C1 Antibody

SKU: orb1925303

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: KO/KD Validated, WB
• Dilution Range: WB - 1:2000
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Clone No.: B4375EV529X54X02
• Immunogen: This antibody is generated from a mouse immunized with a recombinant protein from human.
• Target: NR3C1 (HGNC:7978)
• Molecular Weight: 85659 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL

All lanes: Anti-NR3C1 Antibody at 1:1000 dilution (upper) Lane 1: HeLa Lane 2: HeLa-Knockout Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution. Predicted band size: 85 kDa

All lanes: Anti-NR3C1 at dilution. Lane 1: DU145 whole cell lysate. Lane 2: U-251MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 86 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-NR3C1 at dilution. Lane 1: HeLa whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: CCRF-CEM whole cell lysate. Lane 4: CHO whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 86 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

NR3C1 (HGNC:7978)

UniProt

P04150