NR2F1/Coup-TF Antibody

• Catalog Number: orb1535464
• Category: Antibodies
• Description: NR2F1/Coup-TF Antibody
• Target: NR2F1 / Coup-TF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 1X PBS, pH 7, 0.01% Thimerosal, 10% Glycerol. 1X PBS, pH 7, 0.01% Thimerosal, 10% Glycerol
• Purification: Immunoaffinity purified
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human COUP TF1. The exact sequence is proprietary.
• Tested applications: ICC, IHC, IHC-P, WB
• Dilution range: ICC (1:100 - 1:1000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:3000)
• Application notes: Further information: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NR2F1, COUP-TF1, COUP-TFI, COUP transcription fact
• Note: For research use only

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence of paraformaldehyde-fixed HeLa using COUP TF1 antibody at 1:200 dilution.

Western blot of NR2F1 expression in transfected 293T cell line by NR2F1 polyclonal antibody. A: Non-transfected lysate., B: NR2F1 transfected lysate. 12% SDS PAGE. NR2F1 / Coup-TF antibody diluted at 1:500