NR0B2 Antibody

• Catalog Number: orb1270401
• Category: Antibodies
• Description: NR0B2 Antibody
• Target: NR0B2
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This NR0B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the Central region of human NR0B2.
• UniProt ID: Q15466
• MW: 28 kDa
• Tested applications: FC, IF, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Nuclear receptor subfamily 0 group B member 2, Orp
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: Q15466

Western blot analysis in HepG2 cell line lysates (35 ug/lane).This demondetected the NR0B2 protein (arrow).

NR0B2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of NR0B2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.