NPTX2 Antibody

• Catalog Number: orb1239671
• Category: Antibodies
• Description: NPTX2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Guinea pig
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-NPTX2 antibody (orb1239671) was raised against a peptide corresponding to 16 amino acids near the center of human NPTX2. The immunogen is located within amino acids 170 - 220 of NPTX2.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 47kDObserved: 47 kD
• Target: NPTX2
• UniProt ID: P47972
• NCBI: NP_002514
• Storage: NPTX2 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: NPTX2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: NPTX2 Antibody: NP2, NARP, NP-II, Neuronal pentrax
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Mouse Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: NPTX2 orb1239671 (A: 0.5 µg/mL, B: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: NPTX2 orb1239671 (2 µg/mL), NPTX2 orb1271003 (4 µg/mL), and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: NPTX2 orb1239671 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. A: Absence of blocking peptide B: Presence of blocking peptide.

Immunofluorescence Validation of NPTX2 in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling NPTX2 with orb1239671 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of NPTX2 in Human Brain. Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antibody (orb1239671) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of NPTX2 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with orb1239671 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Immunohistochemistry Validation of NPTX2 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-NPTX2 antibody (orb1239671) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.