NPHS1/Nephrin Antibody

• Catalog Number: orb1535992
• Category: Antibodies
• Description: NPHS1/Nephrin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A 14 AA synthetic peptide near the C-Terminus of human Nephrin. The immunogen is located within the last 50 AA of Nephrin.
• Concentration: 1 mg/ml
• Dilution range: ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 µg/ml)
• Conjugation: Unconjugated
• Target: NPHS1 / Nephrin
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Alternative names: NPHS1, Nephrin, NPHN, CNF
• Note: For research use only
• Application notes: Further information: Nephrin antibody can be used for detection of Nephrin by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 1 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Western Blot:
• Expiration Date: 12 months from date of receipt.

Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 10 ug/ml.

Western blot of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.