NPC1 Antibody

SKU: orb1239337

Description

NPC1 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine
• Application Notes:
  NPC1 antibody can be used for detection of NPC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: NPC1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human NPC1.The immunogen is located within the last 50 amino acids of NPC1.
• Target: NPC1
• Molecular Weight: Predicted: 140 kDa Observed: 150 kDa
• Purification: NPC1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: NPC1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

NPC1 Antibody: NPC, Niemann-Pick C1 protein

Western blot analysis of NPC1 in HepG2 cell lysate with NPC1 antibody at 1 µg/mL.

Immunohistochemistry of NPC1 in mouse kidney tissue with NPC1 antibody at 2.5 µg/mL.

Immunofluorescence of NPC1 in Mouse Kidney cells with NPC1 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

NPC1

UniProt

O15118

RefSeq (Protein):NP_000262