NPC 15199

SKU: orb1298756

Description

FMOC-L-Leucine (NPC 15199) is a small molecule compound recognized for its anti-inflammatory properties. It serves as a useful research tool for investigating inflammatory pathways and mechanisms in both cellular (in vitro) and animal (in vivo) experimental models.

Research Area

Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 35661-60-0
• MW: 353.41
• Purity: 99.94% (May vary between batches)
• Formula: C₂₁H₂₃NO₄
• SMILES: CC(C)C[C@H](NC(=O)OCC1c2ccccc2-c2ccccc12)C(O)=O
• Target: Others
• Solubility: DMSO:25 mg/mL (70.74 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.66 mM)

Bioactivity

• In Vitro:
  NPC-15199 [(N-(9-fluorenylmethoxycarbonyl)L-leucine)], a novel anti-inflammatory agent, increases intracellular Ca2+ concentration ([Ca2+]i) in human bladder female transitional cancer (BFTC) cells. Using fura-2 as a Ca2+ probe, NPC-15199 (0.1-2 mM) was found to increase [Ca2+]i concentration-dependently. The response saturated at 2-5 mM NPC-15199. The [Ca2+]i increase comprised an initial rise, a slow decay, and a plateau. Ca2+ removal partly inhibited the Ca2+ signals. In Ca2+-free medium, pretreatment with 1 mM NPC-15199 abolished the [Ca2+]i increase induced by 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor); and after pretreatment with thapsigargin, NPC-15199-induced Ca2+ release was dramatically inhibited. This indicates that NPC-15199 released internal Ca2+ mostly from the endoplasmic reticulum. Adding 3 mM Ca2+ increased [Ca2+]i in cells pretreated with 1 mM NPC-15199 in Ca2+-free medium. Together, the findings suggest that in BFTC bladder cancer cells, NPC-15199 induced Ca2+ release from the endoplasmic reticulum and activating Ca2+ entry.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Fmoc-leucine, FMOC-L-Leucine, Inhibitor, NSC334290, NSC-334290, inflammation, NSC 334290, NPC 15199, NPC15199, NPC-15199, inhibit, Peroxisome proliferator-activated receptors, PPAR

Compound Identifiers

PubChem CID

1549133