NPC 15199

SKU: orb1219936

Description

NPC 15199 is an anti-inflammatory agent.NPC-15199 [(N-(9-fluorenylmethoxycarbonyl)L-leucine)], a novel anti-inflammatory agent, increases intracellular Ca2+ concentration ([Ca2+]i) in human bladder female transitional cancer (BFTC) cells. Using fura-2 as a Ca2+ probe, NPC-15199 (0.1-2 mM) was found to increase [Ca2+]i concentration-dependently. The response saturated at 2-5 mM NPC-15199. The [Ca2+]i increase comprised an initial rise, a slow decay, and a plateau. Ca2+ removal partly inhibited the Ca2+ signals. In Ca2+-free medium, pretreatment with 1 mM NPC-15199 abolished the [Ca2+]i increase induced by 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor); and after pretreatment with thapsigargin, NPC-15199-induced Ca2+ release was dramatically inhibited. This indicates that NPC-15199 released internal Ca2+ mostly from the endoplasmic reticulum. Adding 3 mM Ca2+ increased [Ca2+]i in cells pretreated with 1 mM NPC-15199 in Ca2+-free medium. Together, the findings suggest that in BFTC bladder cancer cells, NPC-15199 induced Ca2+ release from the endoplasmic reticulum and activating Ca2+ entry.

Research Area

Metabolism Research

Key Properties

• CAS Number: 35661-60-0
• MW: 353.41
• Purity: >98% (HPLC)
• Formula: C₂₁H₂₃NO₄
• SMILES: CC(C)([H])C[C@]([H])(NC(=O)OC[C@@]2([H])c1ccccc1c3ccccc23)C(=O)O
• Target: Anti-inflammatory
• Solubility: In Vitro: DMSO : 50 mg/mL (141.48 mM)

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FMOC-L-Leucine

Quick Database Links

Gene Symbol

Anti-inflammatory