NPAS4 Antibody: APC

• Catalog Number: orb376996
• Category: Antibodies
• Description: Mouse monoclonal antibody against NPAS4 conjugated to APC .
• Target: NPAS4
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: APC
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Immunogen: Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.
• UniProt ID: Q8CJH6
• MW: 90kDa
• Tested applications: ICC, IF, IHC
• Dilution range: WB (1:1000), ICC/IF(1:100)
• Application notes: A 1:100 dilution of SMC-495 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: N408/79 (Formerly sold as S408-79)
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Neuronal PAS domain-containing protein 4 antibody,
• Note: For research use only
• Entrez: 266734
• NCBI: NP_705890.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone N408/79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.

Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone N408/79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 μg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band (s): ~60, 45, 40, 38, 25, 20 kDa.