Cart summary

You have no items in your shopping cart.

NPAS4 Antibody: APC

Catalog Number: orb376996

DispatchUsually dispatched within 2-3 weeks
$ 590.00
Catalog Numberorb376996
CategoryAntibodies
DescriptionMouse monoclonal antibody against NPAS4 conjugated to APC .
Species/HostMouse
ClonalityMonoclonal
Clone NumberN408/79 (Formerly sold as S408-79)
Tested applicationsICC, IF, IHC
ReactivityHuman, Mouse, Rat
IsotypeIgG1
ImmunogenFusion protein amino acids 597-802 (C-terminus) of rat Npas4.
Concentration1 mg/ml
Dilution rangeWB (1:1000), ICC/IF(1:100)
ConjugationAPC
MW90kDa
TargetNPAS4
Entrez266734
UniProt IDQ8CJH6
NCBINP_705890.1
StorageConjugated antibodies should be stored according to the product label
Buffer/Preservatives95.64mM Phosphate, 2.48mM MES and 2mM EDTA
Alternative namesNeuronal PAS domain-containing protein 4 antibody,
Read more...
NoteFor research use only
Application notesA 1:100 dilution of SMC-495 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Expiration Date12 months from date of receipt.
NPAS4 Antibody: APC

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone N408/79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.

NPAS4 Antibody: APC

Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone N408/79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 μg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band (s): ~60, 45, 40, 38, 25, 20 kDa.

  • NPAS4 Rabbit Polyclonal Antibody (APC) [orb998793]

    ICC,  IF

    Bovine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep

    Rabbit

    Polyclonal

    APC

    100 μl
  • NPAS4 Rabbit Polyclonal Antibody (APC-Cy7) [orb2470685]

    ICC,  IF

    Bovine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep

    Rabbit

    Polyclonal

    APC/Cy7

    100 μl
  • NPAS4 Rabbit Polyclonal Antibody (APC-Cy5.5) [orb2470686]

    ICC,  IF

    Bovine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep

    Rabbit

    Polyclonal

    APC/Cy5.5

    100 μl