NOXO1 antibody

• Catalog Number: orb345525
• Category: Antibodies
• Description: NOXO1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: NOXO1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 238-252 of human NOXO1 protein.
• Concentration: 1.0mg/ml
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 5-10 µg/ml, WB: 1:1,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-NOXO-1 antibody is directed against human NOXO1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from human and chimpanzee (100% homology). Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from dog and guinea pig (87%), as well as rat (75%) and mouse (68%). Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q8NFA2
• NCBI: 16198473
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-NOXO1 antibody, NOXO-1, NOXO 1, NADPH
• Note: For research use only
• Application notes: Anti-NOXO1 antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Biorbyt's affinity purified anti-NOXO1 antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of the lamina propia in human colon epithelium and macrophages at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Western blot using Biorbyt's Affinity Purified anti-NOXO1 antibody shows detection of a band ~50 kDa corresponding to human NOXO1. Reactivity was observed in transfected human 293 cells and human HT-29 colon carcinoma cells (endogenous). Under these conditions endogenous NOXO1 detection was not observed in HeLa, HL-60, 3T3, untransfected 293 or WT MEF cells. A 1:1000 dilution of the primary antibody was used for detection followed by secondary antibody reactivity. Specific band reactivity was competed away when the antibody was pre-incubated with the peptide immunogen (data not shown).