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Item 1 of 3
NOX4 Antibody (N-term)
Catalog Number: orb1936206
| Catalog Number | orb1936206 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB34043 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:2000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 66932 Da |
| Target | This NOX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-153 amino acids from the N-terminal region of human NOX4. |
| UniProt ID | Q9NPH5 |
| NCBI | NP_001137308.1, NP_058627.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | NADPH oxidase 4, 163-, Kidney oxidase-1, KOX-1, Ki Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-NOX4 Antibody (N-term) at 1:2000 dilution. Lane 1: Human kidney lysate. Lane 2: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
NOX4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NOX4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes: Anti-NOX4 Antibody (N-term) at 1:2000 dilution. Lane 1: Human kidney lysate. Lane 2: HUVEC whole cell lysate. Lane 3: U-251 MG whole cell lysate. Lane 4: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
